Resumo Esta pesquisa teve como objetivo estimar a relação peso-comprimento (LWR) da arraia Potamotrygon wallacei, conhecida localmente como cururu, que foi capturada em igarapés e lagos na região do médio Rio Negro, Amazonas, Brasil. As arraias foram capturadas durante a noite (das 23h-1h) nas margens dos igarapés e lagos, por meio de busca ativa utilizando canoas de madeira, lanternas de cabeça e puçás. As coletas foram realizadas nos meses de novembro de 2017 (5 dias), fevereiro (8 dias), março (3 dias) e abril (2 dias) de 2018, totalizando 18 dias de amostragem. Foram medidos o peso fresco total com precisão de 0,01 g e a largura do disco com precisão de 0,1 cm. Os parâmetros a e b da equação W= a.DWb foram estimados. Este estudo prover novos dados de comprimento máximo para a arraia cururu.

Abstract This research aimed to estimate the length-weight ratio (LWR) of the stingray Potamotrygon wallacei, known locally as the cururu, which was caught in streams and lakes in the middle Negro River region, Amazonas, Brazil. The stingrays were captured during the night (from 11 pm to 1 am) near the shores of streams and lakes, through active search using wooden canoes, head lanterns and scoop nets. The samplings were carried out in November 2017 (5 days), February (8 days), March (3 days) and April (2 days) of 2018, totaling 18 days of sampling. The total fresh weight was measured to 0.1 g of accuracy and the disc width to 0.1 cm accuracy. The parameters a and b of the equation W=a.DWb were estimated. This study provides new maximum length data for the cururu stingray.

Resumo Este estudo estimou as relações comprimento-peso de 16 espécies de peixes que ocorrem às margens de praias arenosas ao longo da bacia do baixo Rio Negro, Amazônia brasileira. As espécies foram capturadas durante um dia de cada mês, de outubro a novembro de 2016, no início da manhã e no início da noite, usando rede de cerco (20 m de comprimento e 3,5 m de altura, 5 malha mm entre nós opostos). As medidas foram feitas para comprimento padrão (SL - precisão de 0,1 cm) e peso total (TW - precisão de 0,01 g). Os parâmetros a e b da equação WT = a.LTb foram estimados. Os valores de a variaram de 0,0018 a 0,0226 e os valores de b variaram de 2,5271 a 3,3244. Este estudo também fornece novos dados sobre comprimentos máximos para seis espécies, Amazonsprattus scintilla, Brycon pesu, Moenkhausia megalops, Pachyurus paucirastrus, Reganella depressa e Trachydoras microstomus, e novos reportes dos parâmetros da LWRs de 15 espécies de peixes.

Abstract This study estimated the length–weight relationships of 16 fish species occurring close to the shores of sandy beaches along the lower Negro River basin, Brazilian Amazon. The specimens were captured for one day each month, in October to November 2016, early in the morning and early evening, using trawl net (20 m taken for standard length (SL – 0.1 cm precision) and total weight (TW – 0.01 g precision). The parameters a and b of the equation WT = a.LTb were estimated. The a values ranged from 0.0018 to 0.0226 and b values ranged from 2.5271 to 3.3244. This study also provides new data on of maximum lengths for six species, Amazonsprattus scintilla, Brycon pesu, Moenkhausia megalops, Pachyurus paucirastrus, Reganella depressa and Trachydoras microstomus, and new reports of the LWRs parameters of 15 fish species.

Resumo Os peixes que habitam os lagos de várzea da Amazônia apresentam morfologias bastante diversas, possibilitando a exploração bem sucedida dos diferentes habitats que formam estas áreas adjacentes aos grandes rios amazônicos. Neste artigo, relatamos os resultados de uma análise ecomorfológica de vinte espécies abundantes, coletadas em maio e agosto de 2011, em dois lagos das várzeas do trecho inferior do rio Solimões. As analises indicaram diferenças entre as espécies como função do atributos ecomorfológicos, provavelmente associadas com a capacidade natatória e com preferências por habitat.

Abstract Fishes inhabiting Amazonian floodplain lakes exhibits a great variety of body shape, which was a key advantage to colonize the several habitats that compose these areas adjacent to the large Amazon rivers. In this paper, we did an ecomorphological analysis of twenty abundant species, sampled in May and August 2011, into two floodplain lakes of the lower stretch of the Solimões River. The analysis detected differences among species, which could be probably associated with swimming ability and habitat use preferences.

OBJECTIVE: This study reports long-term effects of chronic Achilles tendon rupture treatment, using reconstruction with peroneus brevis transfer (PBT), on sports activities based on an approximate 10-year follow-up study. METHODS: Twenty patients (6 women and 14 men; mean age, 43 ± 12.85 years at the time of operation) underwent chronic Achilles tendon repair with an average follow-up of 164.05 ± 5.07 months. Seven were involved in competitive sports, 10 participated in recreational activities and three were not involved in any sporting activities. All patients were Asians. Results were assessed using Cybex strength testing and the American Othopaedic Foot and Ankle Society (AOFAS) Score, the muscle manual test (MMT), sports activities and comprehensive satisfaction assessment. RESULTS: Cybex strength testing resulted in an average gain of 87.05 ± 14.83% in dorsiflexion strength (range 65-110%) and 98.05 ± 9.02% in plantar flexion strength (range 85%-120%). The AOFAS score average was 86.9 ± 7.27. There were no postoperative re-ruptures, no recurrences and no wound complications. Plantar flexion strength and the AOFAS score were negatively correlated with the age at the time of operation (r = "0.566, r = -0.669, respectively). Seventeen patients (85%) were level five of MMT in eversion strength. Following treatment, six patients (30%) returned to competitive sports, while 10 (50%) who, prior to the injury and surgery, were involved in recreational activities, returned to similar activities. The relatively younger group tended to continue sport activities as competitive athletes (p < 0.05). Significant differences were observed in age at the operation between non-satisfaction group and excellent group (p < 0.05). The under 40-year age group tended to show a poor value. CONCLUSION: Recreational athletes and non-athletes could return to their sports activities satisfactorily, while young competitive athletes found difficulties in certain actions, especially related to eversion.

OBJETIVO: Este estudio reporta efectos a largo plazo del tratamiento de la ruptura crónica del tendón de Aquiles mediante reconstrucción con transferencia del peroneo corto (TPC) en actividades deportivas, sobre la base de un estudio de seguimiento de aproximadamente 10 años. MÉTODOS: Veinte pacientes (6 mujeres y 14 hombres; edad promedio, 43 ± 12.85 años en el momento de la operación) fueron sometidos a una reparación de ruptura crónica del tendón de Aquiles con un seguimiento promedio de 164.05 ± 5.07 meses. Siete estaban en medio de competencias deportivas, 10 participaban en actividades recreativas, y tres estaban fuera de toda actividad deportiva. Todos los pacientes eran los asiáticos. Los resultados se evaluaron usando la prueba de Cybex para medir la fuerza, la puntuación de la escala de la Sociedad Ortopédica Americana de Pie y Tobillo (AOFAS) para la valoración quirúrgica, la prueba muscular manual (PMM), y la evaluación integral de la satisfacción y las actividades deportivas. RESULTADOS: La prueba de Cybex indicó una ganancia promedio de 87.05 ± 14.83% en fuerza de dorsiflexión (rango 65-110%) y 98.05 ± 9.02% en fuerza de flexión plantar (rango 85%-120%). El promedio de la puntuación de la escala de AOFAS fue 86.9 ± 7.27. No se produjeron re-rupturas post-operatorias, ni recurrencias, ni complicaciones de heridas. La fuerza de flexión plantar y la puntuación de la escala AOFAS fueron puestas en correlación negativa con la edad al momento de la operación (r = "0.566, r = -0.669, respectivamente). Diecisiete pacientes (85%) alcanzaron el nivel cinco de la PMM en fuerza de eversión. Tras el tratamiento, seis pacientes (30%) se reintegraron a las competencias deportivas, mientras que 10 (50%) que antes de la lesión y la cirugía participaban en actividades recreativas, se reincorporaron a actividades similares. El grupo relativamente más joven tiende a continuar las actividades deportivas como atletas de competencia (p < 0.05). Se observaron diferencias significativas en edad en la operación entre el grupo sin satisfacción y el grupo excelente (p < 0.05). El grupo por debajo de los 40 años de edad mostró valores más pobres como tendencia. CONCLUSIÓN: Tanto los atletas de actividades recreativas como los no atletas, pudieron regresar a sus actividades deportivas satisfactoriamente, mientras que los competidores jóvenes encontraron dificultades en ciertas acciones, en particular las relacionadas con la eversión.

OBJECTIVE: This study investigated the effect of Low Intensity-pulsed Ultrasound (LIPUS) on the repair process of ruptured Achilles tendon using a rat model and also examined the regulation of a biological molecule that may contribute to this in vitro and in vitro. METHODS: To investigate the effect of LIPUS and its biological mechanism ofpromoting Achilles tendon repair after acute injury, ninety-eight male Sprague-Dawley (SD) rats (mean body weight, 258 ±9.8 g) aged 12 weeks were used in this study. To create the model, the Achilles tendon attachment site and musculotendinous junction were ruptured under direct vision. The leg on one side was exposed to LIPUS (frequency at 1.5 MHz, the repetition cycle at 1.0 kHz, the burst width at 200 msec and the power output at 45 mW/cm2), for 20 minutes daily with a 0.7 mm diameter probe. Results:Low Intensity-pulsed Ultrasound treatment accelerated the repair of the Achilles tendon compared to the untreated group, judged by electron microscopy. Both cyclo-oxygenase (COX)-2* and EP4* expressions were over-expressed in the LIPUS treated group in the inflammatory period, and TGFJ31* expression was markedly induced in LIPUS treated groups followed by collagen I* and III* expression in the repair and reconstitution process. CONCLUSION: These findings suggest that LIPUS is potentially able to accelerate the repair of acute ruptured Achilles tendon in several ways: by exaggerating inflammation by inducing COX-2 and EP4 and reconstituting tissue by inducing TGFJ31 followed by collagen I and III. (*: p < 0.05, **: 0.001)

OBJETIVO: Este estudio estuvo encaminado a investigar el efecto de los ultrasonidos pulsados de baja intensidad (LIPUS) sobre el proceso de reparación del tendón de Aquiles tras una ruptura, usando un modelo de rata. Asimismo, se examinó la regulación de una molécula biológica que puede contribuir a este proceso in vitro e in vitro. MÉTODOS: Con el fin de investigar el efecto de LIPUS y el mecanismo biológico por el cual este efecto promueve la reparación del tendón de Aquiles tras una lesión aguda, noventa y ocho ratas machos Sprague-Dawley (SD) (peso corporal promedio, 258 ± 9.8 g) de 12 semanas de edad fueron usadas en este estudio. Para crear el modelo, el sitio de ligazón microbiológica del tendón de Aquiles y la unión músculo-tendinosa fueron desgarrados bajo visión directa. La pierna de un lado fue expuesta a LIPUS (frecuencia de 1.5 MHz, ciclo de repetición de 1.0 kHz, ancho de ruptura de 200 msec, y potencia de salida de 45 mW/cm2), por 20 minutos diariamente con una sonda de 0.7 mm diámetro. RESULTADOS: El tratamiento de ultrasonidos pulsados de baja intensidad aceleró la reparación del tendón de Aquiles, en comparación con el grupo no tratado, según se apreció mediante el microscopio electrónico. Tanto la ciclo-oxygenasa (COX)-2* como las expresiones EP4* estuvieron sobe-expresadas en el grupo tratado con LIPUS en el periodo inflamatorio, y la expresión TGFfi1* fue marcadamente inducida en los grupos tratados con LIPUS seguidos por la expresión de colágeno I* y III* en el proceso de reparación y reconstitución. CONCLUSIÓN: Estos resultados sugieren que LIPUS puede potencialmente acelerar la reparación del tendón de Aquiles luego de un desgarramiento, de varias maneras: exagerando la inflamación mediante inducción de COX-2 y EP4 y reconstituyendo el tejido induciendo TGFfil seguido por colágeno I y III. (*: p < 0.05, **: 0.001)

OBJECTIVES: This study reports the long-term surgical outcomes of elderly patients who underwent surgery using the modified Hackethal bundle nailing method on the basis of an approximately 10-year follow-up study. METHODS: We treated 34 patients (7 males, 27 females) with 2- and 3-fragment fractures of the proximal humeral neck. Their ages at the time of operation ranged from 65 to 75 years (mean age, 69.5 years). They were classified as Neer group I (G-I, 8.8%), III (G-III, 79.4%) or IV (G-IV, 11.8%). The duration of follow-up in patients averaged 130.6 months (range: 125.0 - 156.0 months). Patients were graded according to the Constant-Murley (CM) scoring system. Pre-operative and postoperative X-rays were also assessed. RESULTS: All the fractures united within 6-9 weeks, with an average of 7.4 weeks. The mean overall Constant score was 80.0points (G-I: 83.3; G-III: 80.5; G-IV: 75.6). Among the 34patients, 30 (88.2%) obtained excellent results and 4 (11.8%) obtained good results. Mal-union in the coronal plane was observed in two patients (6.28%) who had 160 degrees angulation in three part fracture. Mal-union of the greater tuberosity occurred in one patient (3.14%) leading to limitation of abduction to 90 degrees. There were no cases ofavascular necrosis, neurovascular complications or deep infections. CONCLUSION: This study suggests that the modified H-technique is simple, less invasive, and a reliable and effective procedure for elderly patients.

OBJETIVO: Este estudio reporta resultados quirúrgicos a largo plazo de pacientes de edad sometidos a cirugías mediante el método de enclavado en haz de Hacketal modificado, sobre la base de un estudio de seguimiento de aproximadamente 10 años. MÉTODO: Se trataron 34 pacientes (7 varones, 27 hembras) con fracturas en dos y en tres fragmentos del húmero proximal. Sus edades al momento de la operación fluctuaban de 65 a 75 años (edad promedio, 69.5 años). Fueron clasificados como grupo I de Neer (G-I), 8.8%), III (G-III, 79.4%) o IV (G-IV, 11.8%). La duración del seguimiento en los pacientes promedió 130.6 meses (rango: 125.0-156.0 meses). Los pacientes fueron clasificados de acuerdo con el sistema de puntuación Constant-Murley (CM). También se evaluaron los rayos X preoperatorios y postoperatorios. RESULTADOS: Todas las fracturas cicatrizaron en un período de 6 - 9 semanas, para un promedio de 7.4 semanas. El promedio de la puntuación Constant general fue de 80.0 puntos (G-I: 83.3; G-III: 80.5; G-IV: 75.6). De los 34 pacientes, 30 (88.2%) obtuvieron resultados excelentes y 4 (11.8%) obtuvieron buenos resultados. Se observó una mala cicatrizaron en el plano frontal en el caso de dos pacientes (6.28%) que tenían 160 grados de angulación en una factura en tres partes. La mala cicatrización de mayor tuberosidad ocurrió en un paciente (3.14%), y condujo a una limitación de la abducción de 90 adgrados. No se produjeron casos de necrosis avascular, complicaciones neurovasculares o infecciones profundas. CONCLUSIÓN: Este estudio indica que la técnica de Hacketal modificada es simple, menos invasiva, y constituye un procedimiento confiable y efectivo para los pacientes de mayor edad.

OBJECTIVES: In this study, eighteen patients who have had perilunate injury with dislocation or fracture, were evaluated and the patho-mechanics and surgical treatment were studied. SUBJECTS AND METHODS: According to the Green and O 'Brien's classification, type 1 injury occurred in one patient, type 2 in nine, type 4A in five, type 4C in one and type 4D in one. The other one case could not be classified using the Green and O 'Brien's criteria. RESULTS: According to the Evans scoring system, good results were achieved in thirteen patients, fair results, in four, poor results in one and very poor results in none. When the patho-mechanics was esti mated based on the Mayfield's criteria, seventeen patients were classified as stage 3. However, only one case was extremely unusual and should be classified as a subtype of stage 2. CONCLUSION: The results were good in the majority ofpatients who had repair ofperilunate injury.

OBJETIVO: En este estudio, se evaluaron dieciocho pacientes que tuvieron lesión perisemilunar con dislocación o fractura, y se estudiaron la patomecánica y el tratamiento quirúrgico. SUJETOS Y MÉTODO: Sobre la base de la clasificación de Green y O'Brien, se concluye que ocurrieron las siguientes lesiones: lesión de tipo 1 en un paciente, de tipo 2 en nueve, tipo 4A en cinco, tipo 4C en uno y tipo 4D en uno. El otro caso no se pudo clasificar usando criterios de Green y O'Brien. RESULTADOS: El sistema de puntuación de Evans, indica que se lograron buenos resultados en trece pacientes; resultados satisfactorios en cuatro; resultados pobres en uno; resultados muy pobres en ninguno. Cuando se estimó la patomecánica sobre la base de los criterios Mayfield, diecisiete pacientes fueron clasificados como fase 3. Sin embargo, sólo un caso fue sumamente raro y debe ser clasificado como subtipo de fase 2. CONCLUSIÓN: Los resultados fueron buenos en la mayoría de los pacientes que tuvieron reparación de lesión perisemilunar.

Escherichia coli, as a model microorganism, was treated in phosphate-buffered saline under high hydrostatic pressure between 100 and 300 MPa, and the inactivation dynamics was investigated from the viewpoint of predictive microbiology. Inactivation data were curve fitted by typical predictive models: logistic, Gompertz and Weibull functions. Weibull function described the inactivation curve the best. Two parameters of Weibull function were calculated for each holding pressure and their dependence on holding pressure was obtained by interpolation. With the interpolated parameters, inactivation curves were simulated and compared with the experimental data sets.